PCR: Alu Insertion on PV92 Chromosome 16
Students will take their own cheek cells, to extract their own DNA and run gel electrophoresis to compare their Alu insertions to their peers. This lab uses a large amount of biotechnology methods, great for lab based learning.
Research Question:
- What is the genotype for the PV92 Alu insertion on chromosome 16 of the samples in your team?
- What is the frequency of the PV92 element in the class?
Students will:
- Collect a cheek sample for DNA extraction with InstaGene Matrix
- Set up a PCR Reaction using a Thermal Cycler
- Perform Gel Electrophoresis
- Determine the genotype of DNA samples
- Calculate the frequency of PV92 Alu sequence
Lab skills learned:
- DNA Extraction
- PCR Reactions
- Agarose Gel Electrophoresis
- Calculate allele frequency
PCR: Alu Insertion on PV92 Chromosome 16
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