Students perform a genetic transformation in your classroom.
Students transform E.Coli bacteria with a gene that codes for Green Fluorescent Pigment and see their bacteria glow under a blacklight.
The pGLO plasmid has been designed to express green fluorescent pigment (GFP). The GFP gene was first isolated from jellyfish. With advancements in biotechnology, we can now transform E.Coli to express the GFP gene and glow under a green light.
Lab skills learned:
pGLO Lab Manual
Timeline of pGLO Activities
pGLO Teacher Guides
pGLO Student Materials
pGLO Instructional Power Point
pGLO Video Resources
Click here for video resources. These videos are property of Bio-Rad Inc. Our lab protocol may differ from some of the video steps.