Restriction Enzyme Digestion of Lambda DNA

In this lab, students will digest lambda DNA using different restriction enzymes.  The DNA will then be analyzed by Gel Electrophoresis.  

 

Restriction Enzyme Digestion and Analysis of Lambda DNA

Lambda DNA is the DNA of a bacteriophage (bacterial virus).  Bacteriophages attack bacteria by inserting its DNA into the bacterial cell.  Bacteriophage lambda is harmless to humans and other eukaryotic organisms which is why we closely study it!

Research Questions

  • How many HindIII, EcoRI and PStI recognition sites are present on lambda DNA?
  • How long are DNA fragments generated by digestion of lambda DNA with these enzymes?

 Students will

  • Perform a digestion of lambda DNA with HIndIII, EcoRI, and PstI restriction enzymes
  • Load and run digested DNA through Electrophoresis
  • Generate a standard curve based on lambda HindIII DNA Standard
  • Determine length of all DNA fragments by comparing to the standard curve.

Lab skills learned

  • Perform a restriction digest
  • Perform agarose gel electrophoresis
  • Analyze gel electrophoresis

Lambda Digest Instructional Materials- COMING SOON!

Lambda Digest Lab Manual

Lambda Digest Teacher Guides

Lambda Digest Instructional Power Point

Timeline of Lambda Digest Activities

Lambda Digest Student Materials

Lambda Digest Video Resources

Click here for video resources.

These resources are property of Bio-Rad Inc.  Some of our lab protocol may differ from the steps on the video.